Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5176794

Ratcliffe, W A ^[1]; Corrie, J E.T.; Dalziel, A H; Macpherson, J S

---

1. Glasgow Royal Infirmary, United Kingdom

Two direct radioimmunoassays for progesterone in 50 ..mu..L of unextracted serum or plasma with assays involving extraction of serum were compared. The direct assays include the use of either danazol at pH 7.4 or 8-anilino-1-naphthalenesulfonic acid at pH 4.0 to displace progesterone from serum binding-proteins. Progesterone is then assayed by using an antiserum to a progesterone 11..cap alpha..-hemisuccinyl conjugate and the radioligand /sup 125/I-labeled progesterone 11..cap alpha..-glucuronyl tyramine, with separation by double-antibody techniques. Direct assays with either displacing agent gave good analytical recovery of progesterone added to human serum, and progesterone values for patients' specimens correlated well (r > 0.96) with results of assays involving extraction of serum. Precision was similar with each displacing agent over the working range 2.5-100 nmol/L and superior to that of extraction assays. We conclude that these direct assays of progesterone are analytically valid and more robust, precise, and technically convenient than many conventional methods involving extraction of serum.

• OSTI ID: 5176794
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:6
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
PROGESTERONE
RADIOIMMUNOASSAY
BLOOD PLASMA
BLOOD SERUM
IODINE 125
LABELLED COMPOUNDS
PATIENTS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
550601* - Medicine- Unsealed Radionuclides in Diagnostics