Improved enzyme immunoassay for progesterone in human plasma. [Comparison with results of /sup 3/H RIA]
An enzyme immunoassay for progesterone is described in which a progesterone-11..cap alpha..-hemisuccinyl-horseradish peroxidase conjugate is used as the label and an antiserum raised in rabbits to a progesterone-11..cap alpha..-hemisuccinyl-bovine serum albumin conjugate. In this assay, antibody-bound and free steroid are separated by using a second antibody precipitation procedure. The assay has a lower limit of sensitivity of 10pg/assay tube and satisfies the usual criteria of specificity, precision, and accuracy. Results obtained with a comparison radioimmunoassay and our procedure agreed well (r = 0.98). Eighty samples can be assayed per day. It is not only well suited for surveys where accurate determination of progesterone concentrations in small plasma aliquots is required, but also for monitoring ovulation induction in patients attending infertility clinics.
- Research Organization:
- Welsh National School of Medicine, Cardiff, Eng.
- OSTI ID:
- 6549157
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:12; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
IMMUNE SERUMS
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
MAN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PATIENTS
PEROXIDASES
PORPHYRINS
PREGNANES
PRIMATES
PROGESTERONE
RADIOASSAY
RADIOIMMUNOASSAY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES