Area-under-the-curve monitoring of cyclosporine therapy: Performance of different assay methods and their target concentrations

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Therapeutic Drug Monitoring; (USA)

DOI:https://doi.org/10.1097/00007691-199001000-00003· OSTI ID:6945585

Grevel, J; Napoli, K L; Gibbons, S; Kahan, B D ^[1]

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1. Univ. of Texas Medical School, Houston (USA)

The measurement of areas under the concentration-time curve (AUC) was recently introduced as an alternative to trough level monitoring of cyclosporine therapy. The AUC is divided by the oral dosing interval to calculate an average concentration. All measurements are performed at clinical steady state. The initial evaluation of AUC monitoring showed advantages over trough level monitoring with concentrations of cyclosporine measured in serum by the polyclonal radioimmunoassay of Sandoz. This assay technique is no longer available and the following assays were performed in parallel during up to 173 AUC determinations in 51 consecutive renal transplant patients: polyclonal fluorescence polarization immunoassay of Abbott in serum, specific and nonspecific monoclonal radioimmunoassays using {sup 3}H and {sup 125}I tracers in serum and whole blood, and high performance liquid chromatography in whole blood. Both trough levels and average concentrations at steady state measured by those different techniques were significantly correlated with the oral dose. The best correlation (r2 = 0.54) was shown by average concentrations measured in whole blood by the specific monoclonal radioimmunoassay of Sandoz ({sup 3}H tracer). This monitoring technique was also associated with the smallest absolute error between repeated observations in the same patient while the oral dose rate remained the same or was changed. Both allegedly specific monoclonal radioimmunoassays (with {sup 3}H and {sup 125}I tracer) measured significantly higher concentrations than the liquid chromatography.

• OSTI ID: 6945585
• Journal Information: Therapeutic Drug Monitoring; (USA), Journal Name: Therapeutic Drug Monitoring; (USA) Vol. 12:1; ISSN 0163-4356; ISSN TDMOD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOASSAY
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HYDROGEN COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
IMMUNOSUPPRESSIVE DRUGS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
VERTEBRATES