Comparison of cyclosporine determinations in whole blood by three different methods. HPLC, /sup 125/I RIA and /sup 3/H RIA
Journal Article
·
· Am. J. Clin. Pathol.; (United States)
The authors have analyzed and compared the cyclosporine concentrations in whole blood specimens from pediatric renal transplant patients using three different methods: high-performance liquid chromatography (HPLC) (5u C18 reverse-phase column), /sup 3/H radioimmunoassay (RIA), and /sup 125/I RIA (substituted /sup 3/H-tracer in Sandoz Kit with /sup 125/I tracer. Results obtained by the /sup 125/I RIA correlated well with results obtained by the /sup 3/H RIA. Both RIA methods had similar correlation with the HPLC method. The /sup 125/I RIA method showed higher sensitivity and greater precision than the /sup 3/H RIA method. The authors conclude that the /sup 125/I RIA method can be used for cyclosporine determination in whole blood specimens. The use of the /sup 125/I RIA provides a simple and rapid method with higher counting efficiency and less background quenching than the /sup 3/H RIA method, which requires cumbersome liquid scintillation counting procedures.
- Research Organization:
- Children's Hospital of Los Angeles, CA
- OSTI ID:
- 6593308
- Journal Information:
- Am. J. Clin. Pathol.; (United States), Journal Name: Am. J. Clin. Pathol.; (United States) Vol. 4; ISSN AJCPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CONTROL
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
IMMUNOSUPPRESSIVE DRUGS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
QUALITY CONTROL
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CONTROL
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
IMMUNOSUPPRESSIVE DRUGS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
QUALITY CONTROL
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS