Optimized method for measuring cyclosporin A with /sup 125/I-labeled cyclosporin

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5124662

Felder, R A; Mifflin, T E; Bastani, B

We evaluated the use of the new iodinated ligand for the in vitro measurement of cyclosporin A by radioimmunoassay (RIA). Substitution of the iodinated cyclosporin (/sup 125/I-CyA) for the corresponding tritium-labeled analog (/sup 3/H-CyA) considerably simplifies and accelerates the currently available RIA, and improves its precision. Analysis of the respective dose-response curves showed that the 50% B0 value was lower for the /sup 125/I-CyA assay than for the /sup 3/H-CyA assay (37 vs 77 micrograms/L). Use of whole-blood specimens minimized interferences from temperature and hematocrit. We conclude that the use of /sup 125/I-CyA in a commercially available RIA for whole-blood specimens is accessible to most laboratories and provides rapid, reproducible data for management of transplant patients.

• OSTI ID: 5124662
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 7; ISSN CLCHA
• Country of Publication: United States
• Language: English

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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
COMPUTERS
CONTROL
DAYS LIVING RADIOISOTOPES
DRUGS
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RADIOISOTOPES
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TRACER TECHNIQUES
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