CCD(charge-coupled device)-based synchrotron x-ray detector for protein crystallography: Performance projected from an experiment
The intense x radiation from a synchrotron source could, with a suitable detector, provide a complete set of diffraction images from a protein crystal before the crystal is damaged by radiation (2 to 3 min). An area detector consisting of a 40 mm dia. x-ray fluorescing phosphor, coupled with an image intensifier and lens to a CCD image sensor, was developed to determine the effectiveness of such a detector in protein crystallography. The detector was used in an experiment with a rotating anode x-ray generator. Diffraction patterns from a lysozyme crystal obtained with this detector are compared to those obtained with film. The two images appear to be virtually identical. The flux of 10/sup 4/ x-ray photons/s was observed on the detector at the rotating anode generator. At the 6-GeV synchrotron being designed at Argonne, the flux on an 80 x 80 mm/sup 2/ detector is expected to be >10/sup 9/ photons/s. The projected design of such a synchrotron detector shows that a diffraction-peak count >10/sup 6/ could be obtained in approx.0.5 s. With an additional approx.0.5 s readout time of a 512 x 512 pixel CCD, the data acquisition time per frame would be approx.1 s so that ninety 1/sup 0/ diffraction images could be obtained, with approximately 1% precision, in less than 3 min.
- Research Organization:
- Argonne National Lab., IL (USA)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6944892
- Report Number(s):
- CONF-861007-23; ON: DE87004956
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
BREMSSTRAHLUNG
CHARGE-COUPLED DEVICES
COHERENT SCATTERING
CRYSTALLOGRAPHY
DETECTION
DIFFRACTION
ELECTROMAGNETIC RADIATION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
LYSOZYME
MEASURING INSTRUMENTS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PERFORMANCE
PROTEINS
RADIATION DETECTION
RADIATION DETECTORS
RADIATIONS
SCATTERING
SCINTILLATION COUNTERS
SEMICONDUCTOR DEVICES
SYNCHROTRON RADIATION
X-RAY DETECTION
X-RAY DIFFRACTION