Large-aperture CCD x-ray detector for protein crystallography using a fiber-optic taper

Conference · Mon Jul 01 00:00:00 EDT 1991 · Proceedings of SPIE - The International Society for Optical Engineering

DOI:https://doi.org/10.1117/12.45312· OSTI ID:5992273

Strauss, Michael G. ^[1]; Westbrook, Edwin M. ^[1]; Naday, Istvan ^[1]; Coleman, Tom A. ^[1]; Westbrook, Mary L. ^[1]; Travis, Dale J. ^[1]; Sweet, Robert M. ^[2]; Pflugrath, J. W. ^[3]; Stanton, Martin J. ^[4]

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1. Argonne National Laboratory (ANL), Argonne, IL (United States)
2. Brookhaven National Laboratory (BNL), Upton, NY (United States)
3. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (United States)
4. Brandeis Univ., Waltham, MA (United States)

A detector with a 114 mm aperture, based on a charge-coupled device (CCD), has been designed for x-ray diffraction studies in protein crystallography. The detector was tested on a beamline of the National Synchrotron Light Source at Brookhaven National Laboratory with a beam intensity greater than 10⁹ x-ray photons/s. A fiberoptic taper, an image intensifier and a lens demagnify, intensify, and focus the image onto a CCD having 512x512 pixels. A detective quantum efficiency (DQE) of 0.36 was obtained by evaluating the statistical uncertainty in the detector output. The dynamic range of a 4x4 pixel resolution element, comparable in size to a diffraction peak, was 10⁴. The point spread function shows FWHM resolution of approximately 1 pixel, where a pixel on the detector face is 160 fJ.m. A complete data set, consisting of forty-five 1° rotation frames, was obtained in just 36 s of x-ray exposure to a crystal of chicken egg-white lysozyme. In a separate experiment, a lysozyme data set consisting of 495 0.1° frames, was processed by the MADNES data reduction program, yielding symmetry R-factors for the data of 3.2-3.5%. Diffraction images from crystals of the myosin SI head (a= 275 $$\mathring{A}$$) were also recorded. The Bragg spots, only 5 pixels apart, were resolved but were not sufficiently separated to process these data. Changes in the detector design which will improve the DQE and spatial resolution arc outlined. The overall performance showed that this type of detector is well suited for x-ray scattering investigations with synchrotron sources.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5992273
• Report Number(s): ANL/CP--71633; CONF-910294--1; ON: DE91008832
• Journal Information: Proceedings of SPIE - The International Society for Optical Engineering, Journal Name: Proceedings of SPIE - The International Society for Optical Engineering Vol. 1447; ISSN 0277-786X
• Publisher: SPIE
• Country of Publication: United States
• Language: English

Related Subjects

440101* -- Radiation Instrumentation-- General Detectors or Monitors & Radiometric Instruments
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
APERTURES
CHARGE-COUPLED DEVICES
COHERENT SCATTERING
CRYSTALLOGRAPHY
DESIGN
DETECTION
DIFFRACTION
EFFICIENCY
ENZYMES
FIBER OPTICS
GLOBULINS
GLYCOSYL HYDROLASES
HYDROLASES
IMAGE INTENSIFIERS
LYSOZYME
MYOSIN
O-GLYCOSYL HYDROLASES
OPENINGS
ORGANIC COMPOUNDS
PERFORMANCE
PROTEINS
QUANTUM EFFICIENCY
RADIATION DETECTION
RESOLUTION
SCATTERING
SEMICONDUCTOR DEVICES
SPATIAL RESOLUTION
X-RAY DETECTION
X-RAY DIFFRACTION