Metabolism, interactions, and bacterial mutagenicity of nitrogen-containing aromatic hydrocarbons and related chemicals
Microbial mutagenicity assays were combined with high pressure liquid chromatography analysis to evaluate the interactions of binary and complex mixtures of polycyclic aromatic hydrocarbons and related compounds. The maximum mutagenic response was 2083 net revertants per 50 micrograms induced by the direct-acting 2-nitrofluorene, while benzo(a)pyrene induced 345 net revertants at a dose of 25 micrograms per plate with metabolic activation. The direct-acting 2-nitro-7/8-chlorodibenzo-p-dioxin induced 1934 net revertants per 25 micrograms without activation, while 4-nitrobiphenyl induced 1095 net revertants per 250 micrograms with metabolic activation. When binary mixtures of nitro-polychlorinated biphenyls were tested in the mutagenicity assay, the primary interaction observed was inhibition, while synergism was observed with mixtures of nitro-polychlorinated dibenzo-p-dioxins. Binary mixtures of 2-nitro-1,3,7,8-tetrachlorodibenzo-p-dioxin, or pentachlorophenol with benzo(a)pyrene produced synergism, while strictly additive effects were observed with octa- or heptachlorodibenzo-p-dioxin and benzo(a)pyrene mixtures. HPLC analysis of the mixtures indicated that pre-incubation of benzo(a)pyrene with 2-nitro-1,3,7,8-tetrachlorodibenzo-p-dioxin increased the quantity of benzo(a)pyrene-7,8-dihydrodiol-, and 9,10-dihydrodiol metabolites detected.
- Research Organization:
- Texas A and M Univ., College Station, TX (USA)
- OSTI ID:
- 6942801
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
MUTAGEN SCREENING
CHLORINATED AROMATIC HYDROCARBONS
DIOXIN
POLYCYCLIC NITRO COMPOUNDS
BACTERIA
LIQUID COLUMN CHROMATOGRAPHY
METABOLIC ACTIVATION
MIXTURES
REVERTANTS
AROMATICS
CHROMATOGRAPHY
CONDENSED AROMATICS
DISPERSIONS
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
MICROORGANISMS
MUTANTS
NITRO COMPOUNDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
SCREENING
SEPARATION PROCESSES
560300* - Chemicals Metabolism & Toxicology