Oxidative degradation of phenanthrene by the ligininolytic fungus Phanerochaete chrysosporium

Journal Article · Mon Jun 01 04:00:00 EDT 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:6938126

Hammel, K E; Moen, M A ^[1]; Zhigai, Wen; Green, B ^[2]

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1. US Department of Agriculture, Madison, WI (United States)
2. State Univ. of New York, Syracuse (United States)

The ligninolytic fungus Phanerochaete chrysosporium oxidized phenanthrene and phenanthrene-9,10-quinone (PQ) at their C-9 and C-10 positions to give a ring-fission product, 2,2[prime]-diphenic acid (DPA), which was identified in chromatographic and isotope dilution experiments. DPA formation from phenanthrene was somewhat greater in low-nitrogen cultures than in high-nitrogen cultures and did not occur in uninoculated cultures. The oxidation of PQ to DPA involved both fungal and abiotic mechanisms, was unaffected by the level of nitrogen added, and was significantly faster than the cleavage of phenanthrene to DPA. Phenanthrene-trans-9,10-dihydrodiol, which was previously shown to be the principal phenathrene metabolite in nonligninolytic P. chrysosporium cultures, was not formed in the ligninolytic cultures employed here. These results suggest that phenanthrene degradation by ligninolytic P. chrysosporium proceeds in order from phenanthrene [yields] PQ [yields] DPA, involves both ligninolytic and nonligninolytic enzymes, and is not initiated by a classical microsomal cytochrome P-450. The extracellular lignin peroxidases of P. chrysosporium were not able to oxidize phenanthrene in vitro and therefore are also unlikely to catalyze the first step of phenanthrene degradation in vivo. Both phenanthrene and PQ were mineralized to similar extents by the fungus, which supports the intermediacy of PQ in phenanthrene degradation, but both compounds were mineralized significantly less than the structurally related lignin peroxidase substrate pyrene was.

• OSTI ID: 6938126
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:6; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
CARBOHYDRATES
CELL CULTURES
CHROMATOGRAPHY
CONDENSED AROMATICS
ENZYMES
FUNGI
GROWTH
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LIGNIN
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PHENANTHRENE
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES