Metabolism of phenanthrene by Phanerochaete chrysosporium

Journal Article · Fri Nov 01 04:00:00 EST 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5966969

Sutherland, J B; Selby, A L; Freeman, J P; Evans, F E; Cerniglia, C E ^[1]

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1. National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR (United States)

The white rot fungus Phanerochaete chyrsosporium metabolized phenanthrene when it was grown for 7 days at 37C in a medium containing malt extract, D-glucose, D-maltose, yeast extract, and Tween 80. After cultures were grown with (9-{sup 14}C)phenanthrene, radioactive metabolites were extracted from the medium with ethyl acetate, separated by high-performance liquid chromatography, and detected by liquid scintillation counting. Metabolites from cultures grown with unlabeled phenanthrene were identified as phenanthrene trans-9, 10-dihydrodiol, phenanthrene trans-3, 4-dihydrodiol, 9-phenanthrol, 3-phenanthrol, 4-phenanthrol, and the novel conjugate 9-phenanthryl {beta}-D-glucopyranoside. Identification of the compounds was based on their UV absorption, mass, and nuclear magnetic resonance spectra. Since lignin peroxidase was not detected in the culture medium, these results suggest the involvement of monooxygenase and epoxide hydrolase activity in the initial oxidation and hydration of phenanthrene by P. chrysosporium.

• OSTI ID: 5966969
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:11; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BIODEGRADATION
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
ENZYMES
FUNGI
HYDROCARBONS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PHENANTHRENE
PLANTS
PROTEINS
TRACER TECHNIQUES