Bioremediation of aliphatic halocarbons by Phanerochaete chrysosporium

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:491044

Khindaria, A; Grover, T A; Aust, S D ^[1]

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1. Utah State Univ., Logan, UT (United States). Biotechnology Center

Reduced radicals of highly oxidized and electron deficient halocarbons (carbon tetrachloride, chloroform, dichloromethane, trichloroethylene, 1,1,1-trichloroethane) were detected by electron paramagnetic resonance spin trapping spectroscopy in a reductive reaction system consisting of lignin peroxidase, hydrogen peroxide, veratryl alcohol, ethylenediaminetetracetic acid or oxalate, hydrogen peroxide, phenyl-N-t-butylnitrone and 1% of the halocarbon. This is a free radical mediated process which uses the organic acid as the electron donor. To correlate the involvement of this mechanism in vivo, mineralization of trichloroethylene and carbon tetrachloride was studied by ligninolytic cultures of Phanerochaete chrysosporium. Both trichloroethylene and carbon tetrachloride were mineralized by the fungus over a period of nine days. Mineralization of these chemicals did not take place in formaldehyde killed control cultures or nonligninolytic cultures of the fungus indicating that lignin peroxidases play an important role in the process. Since all the components of the reductive system are excreted entracellularly, the authors propose that this fungus can be used for bioremediation of these halocarbons.

• Sponsoring Organization: National Insts. of Health, Bethesda, MD (United States)
• OSTI ID: 491044
• Report Number(s): CONF-9406250--
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
CARBON TETRACHLORIDE
CHLORINATED ALIPHATIC HYDROCARBONS
CHLOROFORM
DATA
FUNGI
REMEDIAL ACTION