Utilization of methanol plus hydrogen by Methanosarcina barkeri for methanogenesis and growth

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6935895

Mueller, V; Blaut, M; Gottschalk, G

Methanosarcina barkeri grew on methanol plus H/sub 2/. Both substrates were consumed in equimolar amounts. Growth was strictly dependently on the presence of acetate, which was required for the biosynthesis of cellular constituents. Only about 0.4% of the methane produced originated from acetate. By using deuterated methanol, it was demonstrated that methanogenesis from this compound under H/sub 2/ did not occur via oxidation of methanol to CO/sub 2/ and subsequent reduction but by direct reduction with H/sub 2/. Growth yields with methanol plus H/sub 2/ and with methanol alone were not significantly different: 2.8 g of cells per mol of methanol in mineral medium and 4.6 g of cells per mol of methanol in complex medium, respectively. Growth of M. barkeri on methanol plus H/sub 2/ depended strictly on the presence of sodium ions in the medium. In the presence of 50 mM K/sup +/ the K/sub s/ for Na/sup +/ was 5 mM.

• Research Organization: Universitaet Goettingen, Germany, F.R.
• OSTI ID: 6935895
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 52:2; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALCOHOLS
ALKANES
ATP
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
DEUTERIUM COMPOUNDS
ELEMENTS
GROWTH
HYDROCARBONS
HYDROGEN
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METHANE
METHANOGENIC BACTERIA
METHANOL
MICROORGANISMS
NONMETALS
NUCLEOTIDES
ORGANIC COMPOUNDS
SYNTHESIS
TRACER TECHNIQUES