Fibroblasts maintain the phenotype and viability of the rat heparin-containing mast cell in vitro
Rat serosal heparin-containing mast cells (HP-MC) were maintained in vitro for as long as 30 days when co-cultured with mouse skin-derived 3T3 fibroblasts. In contrast, when the mast cells were cultured alone, on fibronectin-, gelatin-, or dermal-collagen-coated dishes, on acid and heat-killed fibroblasts in the presence or absence of 24 hr fibroblast-conditioned medium, or on a monolayer of mouse serosal macrophages, they failed to adhere to the dishes, released significant amounts of their histamine and lactate dehydrogenase, and stained with trypan blue, indicating a loss of viability. The rat serosal HP-MC cultured with the 3T3 fibroblasts became so adherent to the fibroblasts that the two cell types could be separated from one another only by trypsinization. The cultured HP-MC stained with both alcian blue and safranin and continued to synthesize proteoglycan at a rate comparable to that of freshly isolated cells. The /sup 35/S-labeled proteoglycan synthesized by these cultured cells, like that produced by freshly isolated rat serosal HP-MC, was a 750,000 to 1,000,000 m.w. proteoglycan containing only heparin glycosaminoglycans of 50,000 to 100,000 m.w. As assessed by electron microscopy, many of the cultured HP-MC resembled freshly isolated cells except that some secretory granules had fused with one another in some cells. These results demonstrate that the in vivo differentiated rat HP-MC maintain their histology, morphology, immunologic responsiveness, histamine content, and ability to synthesize heparin proteoglycan when co-cultured with living fibroblasts.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 6935625
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 135:5; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CULTURES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
DYNAMIC FUNCTION STUDIES
ELECTRON MICROSCOPY
EVEN-ODD NUCLEI
FIBROBLASTS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HEPARIN
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IN VITRO
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MAST CELLS
MICROSCOPY
MOLECULAR WEIGHT
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES
VIABILITY