Fibroblasts induce heparin synthesis in chondroitin sulfate E containing human bone marrow-derived mast cells

Journal Article · Sat Sep 15 04:00:00 EDT 1990 · Blood (Journal of Hematology); (USA)

OSTI ID:6423003

Gilead, L; Bibi, O; Razin, E ^[1]

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1. Hebrew Univ.-Hadassah Medical School, Jerusalem (Israel)

Human bone marrow-derived mast cells (hBMMCs), differentiated in vitro in suspension culture and under the influence of human peripheral blood mononuclear cells conditioned medium (hCM), were tested for their response to recombinant human interleukin-3 (rhIL-3) and for their behavior in different microenvironments. The hBMMCs were incubated in the presence of rhIL-3 and the changes in their proliferation rate were determined. Recombinant hIL-3 induced a more than sixfold increase in 3H-thymidine uptake into the hBMMC DNA in a dose-dependent manner. Human CM used as a control for proliferation response induced a more than eightfold maximal proliferation rate increase. Rabbit anti-rhIL-3 completely inhibited hBMMC 3H-thymidine uptake induced by rhIL-3 and decreased the hCM-induced proliferation by approximately 50%. These hBMMCs were cocultured with four different mytomicin C-treated cell monolayers and assayed for phenotypic changes. After only 2 days in coculture with either embryonic mouse skin-derived fibroblasts (MESFs) or human skin-derived fibroblasts (HSFs), a marked increase in granule number and density was noted on staining with toluidine blue. Mast cells that initially stained alcian blue+/safranin- at day 0 of coculture became alcian blue+/safranin+ during the coculture period. Human BMMC proteoglycan synthesis shifted from approximately 85% chondroitin sulfate E to approximately 60% heparin within 14 to 19 days of coculture with the MESF monolayer and to approximately 50% heparin within 19 days of coculture with the HSF monolayer. None of the above-mentioned changes were noted in cocultures of hBMMCs with 3T3 cell line fibroblast monolayers or in cocultures with bovine vascular endothelium (BVE) cell monolayers.

• OSTI ID: 6423003
• Journal Information: Blood (Journal of Hematology); (USA), Journal Name: Blood (Journal of Hematology); (USA) Vol. 76:6; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CELL DIFFERENTIATION
CHONDROITIN
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
FIBROBLASTS
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEPARIN
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
LYMPHOKINES
MAMMALS
MAN
MAST CELLS
MATERIALS
MITOGENS
MONOCYTES
MUCOPOLYSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHENOTYPE
POLYSACCHARIDES
PRIMATES
PROTEINS
PYRIMIDINES
RABBITS
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SKIN
SOMATIC CELLS
SULFATES
SULFUR COMPOUNDS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES