Mouse bone marrow-derived mast cells (BMMC) change their phenotype when cultured with fibroblasts
The heparin-containing mast cells (HP-MC) that reside in the connective tissues of the mouse, but not the chondroitin sulfate containing mast cells in the gastrointestinal mucosa, stain with safranin when exposed to alcian blue/safranin. Mouse BMMC (the presumptive in vitro counterpart of the in vivo differentiated mucosal mast cell) were cultured for 2-14 days with confluent skin-derived 3T3 fibroblasts in RPMI-1640 containing 10% fetal calf serum and 50% WEHI-3 conditioned medium. Although the BMMC adhered to the fibroblast monolayer, they continued to divide, probably due to the presence of interleukin-3 in the conditioned medium. The mast cells remained viable throughout the period of co-culture, since they failed to release LDG and because they increased their histamine content per cell approx.15-fold. After 8-9 days of co-culture, >50% of the BMMC changed histochemically becoming safranin positive. At this time, 30-50% of the (/sup 35/S)glycosaminoglycans on the proteoglycans synthesized by these co-cultured mass cells were heparin, whereas the initial BMMC synthesized proteoglycans containing only chondroitin sulfate E. That interleukin 3-dependent mouse BMMC can be induced to undergo a phenotypic change so as to express characteristics of a HP-MC suggests that the tissue microenvironment determines the differentiated characteristics of these cells.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 6917098
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADHESION
AMINES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
BONE MARROW
CARBOHYDRATES
CELL CULTURES
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FIBROBLASTS
GLUCOPROTEINS
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEMATOPOIETIC SYSTEM
HEPARIN
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MAST CELLS
MICE
MITOGENS
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHENOTYPE
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
STAINS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES