Inactivation of human T-cell lymphotropic virus, type III by heat, chemicals, and irradiation
Infectivity of human T-cell lymphotropic virus, Type III (HTLV-III) was inactivated by heat more rapidly if in liquid medium than if lyophilized and more rapidly at 60 than 56/sup 0/C. When HTLV-III was added to factor VIII suspension, then lyophilized and heated at 60/sup 0/C for 2 hours or longer there was elimination of 1 X 10(6) in vitro infectious units (IVIU) of virus. Much of the viral inactivation appeared to result from lyophilization. The application of water-saturated chloroform to the lyophilized material containing virus also resulted in elimination of infectivity. HTLV-III was efficiently inactivated by formalin, beta-propiolactone, ethyl ether, detergent, and ultraviolet light plus psoralen. The results are reassuring regarding the potential safety of various biological products.
- Research Organization:
- Food and Drug Administration, Bethesda, MD
- OSTI ID:
- 6934436
- Journal Information:
- Transfusion (Philadelphia); (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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VIRUSES
INACTIVATION
BIOLOGICAL RADIATION EFFECTS
CHLOROFORM
CULTURE MEDIA
HEAT TREATMENTS
INFECTIVITY
LYMPHOCYTES
TEMPERATURE EFFECTS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHLORINATED ALIPHATIC HYDROCARBONS
CONNECTIVE TISSUE CELLS
HALOGENATED ALIPHATIC HYDROCARBONS
LEUKOCYTES
MATERIALS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PARASITES
RADIATION EFFECTS
SOMATIC CELLS
560130* - Radiation Effects on Microorganisms
560200 - Thermal Effects
560300 - Chemicals Metabolism & Toxicology