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The effect of the state of differentiation on labeling of epidermal cell surface glycoproteins

Journal Article · · J. Invest. Dermatol.; (United States)
;
Epidermal cells were grown in a medium in which the Ca++ concentration controlled the stage of differentiation. Cell surface molecules of differentiated and undifferentiated cells were compared by lactoperoxidase-catalyzed iodination, by the interaction with /sup 125/I-lectins, and by the metabolic incorporation of L-(/sup 3/H)-fucose. Molecular weights of the labeled components were determined by SDS-PAGE and autoradiography. After lactoperoxidase iodination, most of the radioactivity was found in polypeptide bands of 79,000, 65,000 and 56,000 daltons. The 79,000 band is the most intense for undifferentiated cells but disappears as differentiation proceeds. The 56,000 band is present in normal cells at all stages of differentiation but is absent from neoplastic cells. Glycoproteins reacted with /sup 125/I-lectins were found at 180,000, 130,000 and 85,000 daltons. The 130,000 band was the most prominent for differentiated cells labeled with wheat germ agglutinin but was essentially absent from the undifferentiated cells. With Ricinus communis agglutinin, this band was weaker for undifferentiated than for differentiated cells but was the most intense for both. After metabolic incorporation of tritiated fucose, radioactive glycoproteins were found at 130,000 and 85,000 daltons, with comparable intensities for differentiated and undifferentiated cells.
Research Organization:
Department of Dermatology, University of Texas Medical Branch, Galveston
OSTI ID:
6933454
Journal Information:
J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 78:5; ISSN JIDEA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM IONS
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHARGED PARTICLES
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
EPIDERMIS
EPITHELIUM
GLUCOPROTEINS
GROWTH
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
METABOLISM
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
QUANTITY RATIO
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SKIN
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES