Alterations in lectin binding to the epidermis following treatment with 8-methoxypsoralen plus long-wave ultraviolet radiation
The alterations in lectin fluorescence stainings to the epidermis were examined in guinea pig skin treated with topical application of a 1% 8-methoxypsoralen (8-MOP) solution plus long-wave ultraviolet (UVA) radiation (1.5-3.5 J/cm2) (PUVA). Serial biopsy specimens taken up to 21 days postirradiation were stained with 8 commercially available lectins labeled with either fluorescein isothiocyanate (FITC) or biotin (followed by avidin D-FITC): Bandeiraea simplicifolia agglutinin I (BSA), concanavalin A (Con-A), Dolichos biflorus agglutinin (DBA), peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA), soybean agglutinin (SBA), Ulex europeus agglutinin I (UEA), and wheat germ agglutinin (WGA). In normal guinea pig skin UEA staining was absent. Following PUVA treatment, UEA and DBA stainings became apparent or stronger in intensity after days 7-14 (UEA) and days 4-7 (DBA), respectively, and returned to negative or weak by days 14-21. Stainings with Con-A, SBA, and WGA gave remarkable decreases in intensity after days 2-4 and recovered to the baseline by days 7-14. Intensity of BSA, PNA, and RCA stainings was decreased to a lesser degree than the other lectins. Such changes were not produced by application of 8-MOP, UVA radiation (less than 10 J/cm2), UVB radiation (900-2700 mJ/cm2), or tape stripping. These results suggest that PUVA treatment perturbs the composition or organization of epidermal cell surface glycoconjugates to induce alterations in lectin stainings.
- Research Organization:
- Kyoto Univ. (Japan)
- OSTI ID:
- 6723446
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 82:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EPIDERMIS
BIOLOGICAL RADIATION EFFECTS
LECTINS
RECEPTORS
BIOPSY
BIOTIN
FLUORESCEIN
GUINEA PIGS
PSORALEN
SKIN
STAINS
ULTRAVIOLET RADIATION
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
AROMATICS
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
COUMARINS
DIAGNOSTIC TECHNIQUES
DRUGS
DYES
ELECTROMAGNETIC RADIATION
EPITHELIUM
HEMATOLOGIC AGENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMIDAZOLES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
RADIATION EFFECTS
RADIATIONS
RODENTS
TISSUES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
560121* - Radiation Effects on Cells- External Source- (-1987)