Lectin binding as a probe of proliferative and differentiative phases in primary monolayer cultures of cutaneous keratinocytes
- Univ. of Michigan, Ann Arbor (USA)
The surface of cells in the cutaneous epidermis of the newborn rat exhibits a discrete change in lectin-binding specificity from Griffonia simplicifolia I-B4 (GS I-B4), specific for {alpha}-D-galactosyl residues, to Ulex europeus agglutinin I (UEA), specific for {alpha}-L-fucose, as the cell leaves the basal layer and differentiates. Primary monolayer cultures of rat keratinocytes maintained in low Ca{sup 2+} medium exhibited a characteristic unimodal pattern in the ratio of bound UEA to bound GS I-B4 (UEA/B4 ratio) over a 7-day culture period as determined by a quantitative fluorometric assay. Estimation of DNA synthesis showed (a) a higher ({sup 3}H)thymidine incorporation when the UEA/B4 ratio was low and (b) a steady but lower incorporation between Days 3 and 4, coincident with the higher UEA/B4 ratio. Autoradiographic results further showed that cells stained intensely with UEA failed to incorporate ({sup 3}H)thymidine into their nuclei. Overall, the results suggest that (a) the increase in the UEA/B4 ratio between Days 2 and 4 reflects the progression of a proportion of the cells in the monolayer to an early spinous cell stage, the ultimate fate of which is desquamation into the medium and (b) the decrease in the UEA/B4 ratio between Days 5 and 7 reflects a consequent proliferative response to this loss of cells.
- OSTI ID:
- 5393329
- Journal Information:
- Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 175:2; ISSN ECREA; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
AZINES
BODY
CALCIUM COMPOUNDS
CELL CULTURES
CELL CYCLE
CELL DIFFERENTIATION
CELL PROLIFERATION
ELECTROPHORESIS
EPIDERMIS
EPITHELIUM
FLUORESCENCE
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LECTINS
LUMINESCENCE
MAMMALS
MICROSCOPY
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SKIN
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES