Development of gastrointestinal surface. VIII. Lectin identification of carbohydrate differences

Journal Article · Fri May 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:5716316

Pang, K Y; Bresson, J L; Walker, W A

Binding of microvillus membranes (MVM) from newborn and adult rats by concanavalin A (Con A), Ulex europaeus (UEA I), Dolichos bifluorus (DBA), and Triticum vulgaris (WGA) was examined to determine the availability of carbohydrate-containing sites for these lectins on the intestinal surface during development. Consistent patterns of differences in the reaction of MVM with these lectins were found. Con A and UEA had much higher reactivities to MVM of adult than newborn rats. /sup 125/I-labeled-UEA gel overlay experiments revealed the abundance of UEA-binding sites in MVM of adult rat in contrast to the two binding sites in MVM of a newborn rat. DBA bound only to MVM of the adults, and very few binding sites were found in immature MVM. In contrast to these lectins, WGA binding was much higher in MVM of the newborns and decreased with maturation. Additional experiments on the age dependence of UEA and DBA reactivities revealed that the most striking changes occur in animals from 2 to 2 wk of age. In MVM from 2-wk-old rats, there were only 13.9% and < 0.2% of the adult binding capacities for UEA and DBA, respectively. By the time the animals were 4 wk old, the binding capacity for UEA had attained close to the level of the adults, whereas for DBA it reached 71.3% of the adult value. These results provide definite evidence of changes in the intestinal surface during perinatal development.

• Research Organization: Univ. of California, Los Angeles, Torrance
• OSTI ID: 5716316
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:5; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADULTS
AGE DEPENDENCE
AGE GROUPS
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LECTINS
MAMMALS
MEMBRANES
MUCOUS MEMBRANES
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ONTOGENESIS
RADIOISOTOPES
RATS
RODENTS
UPTAKE
VERTEBRATES