Sialoglycoproteins of murine RAW117 large cell lymphoma/lymphosarcoma sublines of various metastatic colonization properties

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(86)90594-X· OSTI ID:5785389

Irimura, T; Tressler, R J; Nicolson, G L

A metastatic model for large-cell lymphoma/lymphosarcoma has been developed by sequential selection in vivo of the murine RAW117 cell line for enhanced liver metastasis or in vitro for loss of lectin-binding properties. The metastatic variants obtained from such selections show alterations in cell surface lectin-binding components, such as the wheat germ agglutinin (WGA)-reactive sialoglycoproteins. Detergent lysates from RAW117 cells were analyzed by polyacrylamide gel electrophoresis (PAGE) followed by reaction with /sup 125/I-labeled WGA. The (/sup 125/I)WGA became bound to a diffuse band of M/sub r/ 120,000-200,000 in the gels that overlapped with the major sialoglycoprotein band revealed by the periodate-sodium borotritide labeling. However, the (/sup 125/I)WGA reactivity diminished when gels were pretreated with mild acid to remove sialic acid in situ. The binding of (/sup 125/I)WGA to the glycoprotein(s) was greater in the high liver-colonizing RAW117--H10 subline than in the parental RAW117-P line. Another lectin with different saccharide specificity, Ricinus communis agglutinin I (RCA/sub I/), became bound to a similar class of sialoglycoproteins, as well as to glycoproteins of lower M/sub r/, but only when the gels were pretreated with mild acid to remove sialic acid. /sup 125/)WGA reactivity to the sialoglycoprotein components, before and after Smith degradation in situ, strongly suggests that the oligosaccharide back-bones are highly branched and asparagine-linked.

• Research Organization: Univ. of Texas M. D. Anderson Hospital and Tumor Institute, Houston
• OSTI ID: 5785389
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 165; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GLUCOPROTEINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LYMPHOMAS
LYMPHOSARCOMAS
MAMMALS
MEMBRANES
METASTASES
MICE
MONOSACCHARIDES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RODENTS
SACCHARIDES
SARCOMAS
SIALIC ACID
TRACER TECHNIQUES
VERTEBRATES