Sialoglycoproteins of murine RAW117 large cell lymphoma/lymphosarcoma sublines of various metastatic colonization properties
A metastatic model for large-cell lymphoma/lymphosarcoma has been developed by sequential selection in vivo of the murine RAW117 cell line for enhanced liver metastasis or in vitro for loss of lectin-binding properties. The metastatic variants obtained from such selections show alterations in cell surface lectin-binding components, such as the wheat germ agglutinin (WGA)-reactive sialoglycoproteins. Detergent lysates from RAW117 cells were analyzed by polyacrylamide gel electrophoresis (PAGE) followed by reaction with /sup 125/I-labeled WGA. The (/sup 125/I)WGA became bound to a diffuse band of M/sub r/ 120,000-200,000 in the gels that overlapped with the major sialoglycoprotein band revealed by the periodate-sodium borotritide labeling. However, the (/sup 125/I)WGA reactivity diminished when gels were pretreated with mild acid to remove sialic acid in situ. The binding of (/sup 125/I)WGA to the glycoprotein(s) was greater in the high liver-colonizing RAW117--H10 subline than in the parental RAW117-P line. Another lectin with different saccharide specificity, Ricinus communis agglutinin I (RCA/sub I/), became bound to a similar class of sialoglycoproteins, as well as to glycoproteins of lower M/sub r/, but only when the gels were pretreated with mild acid to remove sialic acid. /sup 125/)WGA reactivity to the sialoglycoprotein components, before and after Smith degradation in situ, strongly suggests that the oligosaccharide back-bones are highly branched and asparagine-linked.
- Research Organization:
- Univ. of Texas M. D. Anderson Hospital and Tumor Institute, Houston
- OSTI ID:
- 5785389
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 165
- Country of Publication:
- United States
- Language:
- English
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