Canine renal parathyroid hormone receptor is a glycoprotein: characterization and partial purification

Journal Article · Tue Dec 01 04:00:00 EST 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00398a044· OSTI ID:5436067

Karpf, D B; Arnaud, C D; King, K; Bambino, T; Winer, J; Nyiredy, K; Nissenson, R A

Covalent labeling of the canine renal parathyroid hormone receptor with (/sup 125/I)bPTH(1-34) reveals several major binding components that display characteristic consistent with a physiologically relevant adenylate cyclase linked receptor. Through the use of the specific glycosidases neuraminidase and endoglycosidase F and affinity chromatography on lectin-agarose gels, we show here that the receptor is a glycoprotein that contains several complex N-linked carbohydrate chains consisting of terminal sialic acid and penultimate galactose in a ..beta..1,4 linkage to N-acetyl-D-glucosamine. No high mannose chains or O-linked glycans appear to be present. The peptide molecular weight of the deglycosylated labeled receptor is 62,000 (or 58,000 if the mass of bPTH(1-34) is excluded). The binding of (/sup 125/I)bPTH(1-34) to the receptor is inhibited in a dose-dependent fashion by wheat-germ agglutinin, but not by either succinylated wheat-germ agglutinin or Ricinus communis lectin, suggesting that terminal sialic acid may be involved in agonist binding. A combination of lectin affinity chromatography and immunoaffinity chromatography affords a 200-fold purification of the covalently labeled receptor.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5436067
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:24; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENDOCRINE GLANDS
GLANDS
GLUCOPROTEINS
HALIDES
HALOGEN COMPOUNDS
HALOGENATION
HORMONES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINATION
IODINE 125
IODINE 131
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARATHORMONE
PARATHYROID GLANDS
PEPTIDE HORMONES
PHOSPHORS
PROTEINS
PURIFICATION
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SACCHARIDES
SODIUM COMPOUNDS
SODIUM IODIDES
TRACER TECHNIQUES
VERTEBRATES