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An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts

Journal Article · · Anat. Rec.; (United States)
;
Analysis of electron microscopic radioautographs revealed a maximum labeling with /sup 3/H-proline of rough endoplasmic reticulum (RER) at 3 minutes, Golgi saccules 1 and 2 at 10 minutes, Golgi saccules type 3 at 20 minutes, and presecretory and secretory granules at 30 minutes. Labeling of the extra-cellular collagen matrix occurred at 30 minutes and increased with time. These observations suggest that pro-a-chains of collagen in periodontal ligament fibroblasts are synthesized in the RER and transported to the Golgi apparatus within 10 minutes. These chains then undergo parallel alignment in Golgi saccules type 2 and form segment-long-spacing-like crystallites in Golgi saccules type 3 between 10 and 20 minutes. The peak labeling of presecretory granules and mature secretory granules in small amounts at 30 minutes and the rapid increase in labeling of extracellular collagen matrix which begins at 30 minutes, indicates that the formation of secretory granules requires approximately 30 minutes and that a rapid system of secretory granule translocation exists in periodontal ligament fibroblasts. This evidence further supports the previously published morphologic evidence for a microtubule-dependent system of collagen secretion in periodontal ligament fibroblasts (Cho and Garant, 1981b).
Research Organization:
Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook
OSTI ID:
6933314
Journal Information:
Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 201:4; ISSN ANREA
Country of Publication:
United States
Language:
English

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Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZOLES
BIOCHEMISTRY
BODY
CARBOXYLIC ACIDS
CHEMISTRY
COLLAGEN
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
ELECTRON MICROSCOPY
FIBROBLASTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGAMENTS
MAMMALS
MICE
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYSIOLOGY
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RODENTS
SCLEROPROTEINS
SECRETION
SOMATIC CELLS
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES