Radioautographic characterization of successive compartments along the rough endoplasmic reticulum-Golgi pathway of collagen precursors in foot pad fibroblasts of (/sup 3/H)proline-injected rats
Journal Article
·
· J. Cell Biol.; (United States)
Young rats given an intravenous injection of (/sup 3/H)proline were killed at successive times from 4 to 80 min later. Fibroblasts from the front foot pad were radioautographed; silver grains were counted over several of the organelles and the results were expressed as percent radiolabel per unit volume. These percentages reached a peak over rough endoplasmic reticulum cisternae at 4 min, intermediate vesicles and tubules at 10 min, spherical distensions of cis-side Golgi saccules at 20 min, cylindrical distensions of trans-side saccules between 40 and 60 min, and secretory granules at 60 min. It is proposed that the succession of peaks corresponds to the migration pathway of collagen precursor proteins within fibroblasts; that is, the proteins synthesized in rough endoplasmic reticulum are delivered by intermediate vesicles and/or tubules to the spherical distensions of cis-side saccules, somehow pass from there to the cylindrical distensions of trans-side saccules and, finally, are carried by secretory granules to the extracellular space. 37 references, 3 figures, 2 tables.
- Research Organization:
- McGill Univ., Montreal, Quebec
- OSTI ID:
- 6029155
- Journal Information:
- J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 98:5; ISSN JCLBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZOLES
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BODY
BODY AREAS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
COLLAGEN
CONNECTIVE TISSUE CELLS
FEET
FIBROBLASTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGS
LIMBS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRECURSOR
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RATS
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZOLES
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BODY
BODY AREAS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
COLLAGEN
CONNECTIVE TISSUE CELLS
FEET
FIBROBLASTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGS
LIMBS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRECURSOR
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RATS
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES