An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: II. Colchicine-treated fibroblasts

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Anat. Rec.; (United States)

DOI:https://doi.org/10.1002/ar.1092010403· OSTI ID:6933301

Cho, M I; Garant, P R

Colchicine administered intravenously depolymerized microtubules and disrupted the normal organization of the Golgi apparatus in periodontal ligament fibroblasts. Radioautography with /sup 3/H-proline indicated that collagen secretion was completely inhibited during a period of approximately 4 hours following the onset of the colchicine effect. During this period of secretory inhibition, labeled collagen precursors were present within a variety of dense bodies, primarily located in a juxtanuclear location replacing the normal Golgi complex. The time course of /sup 3/H-proline labeling from 2 to 8 hours suggested that small, newly formed dense bodies fused to form larger dense bodies and pleomorphic structures (zebra bodies), within which collagen precursors appeared to undergo partial polymerization. Autophagosomes, many labeled with /sup 3/H-proline, also increased in number after colchicine administration. A gradual decline in /sup 3/H-proline label occurred from 4 to 24 hours, presumably due to exocytosis of dense bodies or by the digestion of labeled collagen precursors within autophagosomes. These results support the concept that an intact microtubular network is essential for the organized transport of collagen precursors, from the rough endoplasmic reticulum to the Golgi apparatus, and the eventual transport and exocytosis of collagen secretory granules.

• Research Organization: Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook
• OSTI ID: 6933301
• Journal Information: Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 201:4; ISSN ANREA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
ANIMAL CELLS
ANIMAL TISSUES
ANTIMITOTIC DRUGS
ANTIPYRETICS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOSYNTHESIS
BODY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMISTRY
COLCHICINE
COLLAGEN
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DRUGS
FIBROBLASTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGAMENTS
ORGANIC COMPOUNDS
PHYSIOLOGY
PRECURSOR
PROTEINS
SCLEROPROTEINS
SECRETION
SOMATIC CELLS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS