Deposition of idiotype-anti-idiotype immune complexes in renal glomeruli after polyclonal B cell activation

Journal Article · Sat May 01 04:00:00 EDT 1982 · J. Exp. Med.; (United States)

DOI:https://doi.org/10.1084/jem.155.5.1385· OSTI ID:6932695

Goldman, M; Rose, L M; Hochmann, A; Lambert, P H

We investigated the possible role of idiotypic interactions in the pathogenesis of the glomerular lesions observed in mice undergoing polyclonal B cell activation. BALB/c mice were studied for the presence of renal deposits of T15 idiotype-anti-T15 idiotype-immune complexes (IC) after injection of bacterial lipopolysaccharides (LPS). The T15 idiotype is the major idiotype of BALB/c mice anti-phosphorylcholine (PC) antibodies, which are cross-reactive with the idiotype of the TEPC-15 myeloma protein. This model was used because T15 idiotype-anti-T15 idiotype IC have been detected in the circulation of BALB/c mice after polyclonal B cell activation. First, an idiotype-specific immunofluorescence technique allowed us to detect T15 idiotype-bearing immunoglobulins in glomeruli from day 6 to day 28 after LPS injection. Second, fluorescein isothiocyanate-conjugated TEPC-15 myeloma protein was found to localize in the glomeruli after in vivo injection 18 d after LPS administration. This renal localization was shown to be idiotype-specific and could be quantified in a trace-labeling experiment. Third, kidney-deposited immunoglobulins of mice injected with LPS were eluted, radiolabeled, and analyzed by radioimmunoassay. Both T15 idiotype-bearing immunoglobulins and anti-T15 idiotype antibodies were detected in the eluates, providing further evidence for a renal deposition of T15 idiotype-anti-T15 idiotype IC. Polyclonal B cell activation is likely to result in a simultaneous triggering of many idiotypic clones and of corresponding anti-idiotypic clones represented in the B cell repertoire. This could lead to the formation of a variety of idiotype-anti-idiotype IC that could participate in the development of glomerular lesions.

• Research Organization: World Health Organization Immunology Research and Training Center, Hopital Cantonal, Geneva, Switzerland
• OSTI ID: 6932695
• Journal Information: J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 155:5; ISSN JEMEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ABSCESSES
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BIOCHEMISTRY
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
GLOBULINS
GLOMERULI
IMMUNE REACTIONS
IMMUNOGLOBULINS
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PATHOLOGY
PHYSIOLOGY
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES