Evaluation of discrete cellulase enzyme activities from anaerobic digester sludge fed a municipal solid waste feedstock
- National Renewable Energy Lab., Golden, CO (United States)
Anaerobic digestion of municipal solid waste (MSW) represents a waste disposal option that results in the production of a gaseous fuel (methane) and an organic residue suitable for use as a soil amendment. The rate-limiting step in this process is the hydrolysis of polymeric substrates, such as cellulose. However, only limited information exists concerning the hydrolytic capacity of anaerobic digestion systems. The authors have previously reported methods for extracting active hydrolytic enzymes from digester sludge solids using a variety of detergents. Additionally, they used these protocols to quantitatively recover active digester-resident enzymes to determine their operational optima for activity. In this study, they developed new protocols to partially purify endoglucanase enzymes from extracted anaerobic digester sludge fed a MSW feedstock. Fractions from preparative, native polyacrylamide gel electrophoresis (PAGE) were analyzed for endoglucanase activity using carboxymethyl cellulose (CMC) hydrolysis and zymogram activity staining. Finally, a fraction of the total endoglucanase enzyme from this system was evaluated for apparent mol wt using size exclusion chromatography, thus developing a preliminary picture of the cellulase system type.
- OSTI ID:
- 6929318
- Journal Information:
- Applied Biochemistry and Biotechnology; (United States), Vol. 45-46; ISSN 0273-2289
- Country of Publication:
- United States
- Language:
- English
Similar Records
Proteomic and Functional Analysis of the Cellulase System Expressed by Postia placenta during Brown Rot of Solid Wood
The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
CELLULASE
FRACTIONATION
METHANE
BIOSYNTHESIS
MUNICIPAL WASTES
ANAEROBIC DIGESTION
SOLID WASTES
ALKANES
BIOCONVERSION
DIGESTION
ENZYMES
GLYCOSYL HYDROLASES
HYDROCARBONS
HYDROLASES
MANAGEMENT
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PROCESSING
PROTEINS
SEPARATION PROCESSES
SYNTHESIS
WASTE MANAGEMENT
WASTE PROCESSING
WASTES
090900* - Biomass Fuels- Processing- (1990-)
550200 - Biochemistry
320604 - Energy Conservation
Consumption
& Utilization- Municipalities & Community Systems- Municipal Waste Management- (1980-)