Anaerobic digestion of municipal solid waste: Analysis of cellulose biodegradative power
- National Renewable Energy Lab., Golden, CO (United States)
Anaerobic digestion of municipal solid waste (MSW) represents a waste disposal option which results in the production of a gaseous fuel (methane) and an organic residue suitable for use as a soil amendment. The rate limiting step in this process is the hydrolysis of polymeric substrates such as cellulose. Analysis of digester sludge resident cellulase enzyme activity will be discussed. Cellulase enzyme activities are removed from sludge solids by a detergent extraction protocol. The analysis of discrete cellulase activities was accomplished using non-denaturing gel electrophoresis and Zymogram activity staining for CMC activity. Preliminary isolations of discrete cellulase activities from anaerobic digester sludge was performed by preparation isoelectric focussing using the Rainin RF-3 system.
- OSTI ID:
- 141967
- Report Number(s):
- CONF-930304--
- Country of Publication:
- United States
- Language:
- English
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