Cellulase production by the anaerobic digestion process
An anaerobic digestion process is described for the production of cellulolytic enzymes using a methanogenic cellulose-enrichment culture. After a heat treatment designed to destroy all but spore-forming bacteria, this culture produced cellulase from a variety of cellulosic materials as well as from cellobiose. The enzyme system contained endo- and exoglucanase, acted on filter paper, and showed cellobiase and xylanase activities. It was stable at 2/sup 0/C under aerobic conditions and showed a pH optimum at 5 and a temperature optimum at 50/sup 0/C. Endoglucanase and filter paper activities were mostly exogenic, whereas cellobiase and xylanase activities were cell associated. The cellulolytic activity produced by this mixed culture was comparable to that of commercially available fungal preparations, and the process could be useful as an alternate source for these enzymes.
- Research Organization:
- National Research Council of Canada, Ottawa, Ontario
- OSTI ID:
- 5355718
- Report Number(s):
- CONF-810554-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 11; ISSN BIBSB
- Country of Publication:
- United States
- Language:
- English
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ANAEROBIC DIGESTION
BIOCONVERSION
CARBOHYDRATES
CELLULASE
CELLULOSE
DIGESTION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
MANAGEMENT
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROCESSING
PRODUCTION
SACCHARIDES
WASTE MANAGEMENT
WASTE PROCESSING