Production of cellulase, beta-glucosidase, and xylanase by schizophyllum commune grown on a cellulose-peptone medium
The carboxymethylcellulase activity in a S. commune culture containing optimal amounts of cellulose and peptone was 52.4 units/ml at pH approximately 5 and temperature 30 degrees. Enzymatic activities of the optimized S. commune culture filtrate were 1.32 and 26.5 units/ml for filter paper cellulase and cellobiase respectively, at 50 degrees. Other enzymatic activities at 30 degrees were: avicelase 0.02, cellobiase 17.9, p-nitrophenyl-beta-glucosidase 12.5, and xylanase 203.0 units/ml. The S. commune enzyme complex with its high cellobiase and low avicelase activities appears to be complementary to the enzyme complex of Trichoderma reesei. Thus, a mixed fungal culture filtrate may be best for cellulose saccharification.
- Research Organization:
- Pulp and Paper Research Inst Canada, Pointe Claire, PQ Canada H9R 3J9
- OSTI ID:
- 7072005
- Journal Information:
- Dev. Ind. Microbiol.; (United States), Journal Name: Dev. Ind. Microbiol.; (United States) Vol. 22; ISSN DIMCA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
FUNGI
GLUCOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHARIFICATION
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
TRICHODERMA VIRIDE
XYLANASE