Binucleate cell formation correlates to loss of colony-forming ability in X-irradiated cultured mammalian cells

Journal Article · Mon Dec 01 04:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576922· OSTI ID:6928402

Sasaki, H; Yoshinaga, H; Kura, S

The relationship between binucleate cell formation and the loss of colony-forming ability was examined in several cultured mammalian cell lines irradiated with X rays. The maximum fraction of binucleate cells after X irradiation increased dose-dependently within the range in which reproductive cell death might predominate over interphase cell death. When the logarithm of percentage survival was plotted against the percentage binucleate cells, a similar correlation was found for all cell lines tested, with the exception of mouse leukemia L5178Y cells, the most radiosensitive cells used. These observations suggest that the fraction of binucleate cells in the cell population can serve as a measure of cellular radiation damage.

• Research Organization: Kyushu Univ., Fukuoka, Japan
• OSTI ID: 6928402
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CELL CONSTITUENTS
CELL CULTURES
CELL DIVISION
CELL NUCLEI
COLONY FORMATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
RADIATIONS
RADIOSENSITIVITY
SURVIVAL TIME
TUMOR CELLS
X RADIATION