Pyrimidine dimer dependent cleavage of single-stranded DNA by T4 UV endonuclease
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
T4 UV endonuclease cleaves double- and single-stranded DNA with equal specificity for photo-pyrimidine dimers. Thus, the enzyme can be used for mapping and quantifying pyrimidine dimers in single-stranded DNA as well as in double-stranded DNA. Mapping of pyrimidine dimers shows that rates of UV-dimerization are not only affected by 5', 3' adjacent bases, but also by position within pyrimidine tracts. Di-pyrimidines at 3' ends of tracts are more photoreactive than those at 5' ends.
- Research Organization:
- Univ. of Minnesota Medical School, Minneapolis
- OSTI ID:
- 6928398
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus uv-specific endonucleases
DENV gene of bacteriophage T4 codes for both pyrimidine dimer-DNA glycosylase and apyrimidinic endonuclease activities
Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase
Journal Article
·
Wed Dec 24 23:00:00 EST 1980
· J. Biol. Chem.; (United States)
·
OSTI ID:6055809
DENV gene of bacteriophage T4 codes for both pyrimidine dimer-DNA glycosylase and apyrimidinic endonuclease activities
Journal Article
·
Thu Oct 01 00:00:00 EDT 1981
· J. Virol.; (United States)
·
OSTI ID:5927276
Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase
Journal Article
·
Mon May 01 00:00:00 EDT 1989
· J. Bacteriol.; (United States)
·
OSTI ID:6073653
Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLEAVAGE
CRYSTAL STRUCTURE
DNA
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYMES
ESTERASES
GENETIC EFFECTS
GENETIC MAPPING
GENETIC RADIATION EFFECTS
HYDROLASES
MAPPING
MICROORGANISMS
MICROSTRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLEAVAGE
CRYSTAL STRUCTURE
DNA
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYMES
ESTERASES
GENETIC EFFECTS
GENETIC MAPPING
GENETIC RADIATION EFFECTS
HYDROLASES
MAPPING
MICROORGANISMS
MICROSTRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES