Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6073653
The role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. T4 denV phage were equally sensitive when plated on wild-type E. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as T4 pyrimidine dimer-DNA glycosylase. A uvrA mutant of E. coli in which the repair of pyrimidine dimers was dependent on the T4 denV gene carried on a plasmid was constructed. Neither an xth nor an nfo derivative of this strain was more sensitive than the parental strain to UV irradiation. We were unable to construct a uvrA xth nfo triple mutant. In addition, T4, which turns off the host UvrABC excision nuclease, showed reduced plating efficiency on an xth nfo double mutant.
- Research Organization:
- State Univ. of New York, Albany (USA)
- OSTI ID:
- 6073653
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 171:5; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Journal Article
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Thu Oct 01 00:00:00 EDT 1981
· J. Virol.; (United States)
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Site-directed mutagenesis of the T4 endonuclease V gene: role of tyrosine-129 and -131 in pyrimidine dimer-specific binding
Journal Article
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Mon Mar 21 23:00:00 EST 1988
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·
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Journal Article
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Thu Dec 31 23:00:00 EST 1981
· J. Virol.; (United States)
·
OSTI ID:6723184
Related Subjects
560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYMES
ESCHERICHIA COLI
ESTERASES
FUNCTIONS
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MUTATIONS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SURVIVAL CURVES
ULTRAVIOLET RADIATION
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYMES
ESCHERICHIA COLI
ESTERASES
FUNCTIONS
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MUTATIONS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SURVIVAL CURVES
ULTRAVIOLET RADIATION
VIRUSES