Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase
The role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. T4 denV phage were equally sensitive when plated on wild-type E. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as T4 pyrimidine dimer-DNA glycosylase. A uvrA mutant of E. coli in which the repair of pyrimidine dimers was dependent on the T4 denV gene carried on a plasmid was constructed. Neither an xth nor an nfo derivative of this strain was more sensitive than the parental strain to UV irradiation. We were unable to construct a uvrA xth nfo triple mutant. In addition, T4, which turns off the host UvrABC excision nuclease, showed reduced plating efficiency on an xth nfo double mutant.
- Research Organization:
- State Univ. of New York, Albany (USA)
- OSTI ID:
- 6073653
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 171:5; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYMES
ESCHERICHIA COLI
ESTERASES
FUNCTIONS
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MUTATIONS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SURVIVAL CURVES
ULTRAVIOLET RADIATION
VIRUSES