Studies on the biodisposition of organophosphates in mice. Annual summary report No. 2, 1 August 1983-31 July 1984

Technical Report · Wed Aug 01 04:00:00 EDT 1984

OSTI ID:6924498

Martin, B R

The correlation between the biodisposition of radiolabeled organophosphates and their pharmacological effects were studied in mice after either i.v. administration or inhalation. It was found that /sup 3/H-diisopropylfluoro-phosphate (DFP) penetrated all tissues within 1 min of i.v. treatment. Irreversible binding to tissues occurred immediately. There was also evidence that appreciable quantities of /sup 3/H-DFP were rapidly hydrolyzed to /sup 3/H-DIP. Cholinesterase inhibition in plasma, diaphragm and brain were temporally correlated with the levels of bound /sup 3/H-DIP except at some of the earlier time points. DFP also produced a decrease in spontaneous activity, the time course of which was correlated with neither /sup 3/H-DFP and /sup 3/H-DIP levels nor cholinesterase inhibition in brain which suggested that noncholinesterase bound /sup 3/H-DIP was responsible for this CNS depression.

• Research Organization: Medical Coll. of Virginia, Richmond (USA). Dept. of Pharmacology and Toxicology
• OSTI ID: 6924498
• Report Number(s): AD-A-194523/7/XAB
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKYL RADICALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLESTERASES
CHEMICAL REACTIONS
CHOLINESTERASE
DECOMPOSITION
DOCUMENT TYPES
ENZYMES
ESTERASES
HYDROGEN ISOTOPES
HYDROLASES
HYDROLYSIS
INHIBITION
IRREVERSIBLE PROCESSES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MAMMALS
METABOLISM
METHYL RADICALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHARMACOLOGY
PHOSPHONIC ACIDS
PROGRESS REPORT
RADICALS
RADIOISOTOPES
RODENTS
SOLVOLYSIS
TOXICITY
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES