Modification of collagen and noncollagenous proteins in radiation-induced muscular fibrosis

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Exp. Mol. Pathol.; (United States)

DOI:https://doi.org/10.1016/0014-4800(88)90064-0· OSTI ID:6922606

Wegrowski, J; Lafuma, C; Lefaix, J L; Daburon, F; Robert, L

Six months after acute local gamma irradiation of the pig skin and adjacent muscle, the muscular tissue is replaced by a large mutilating and proliferative fibrosis deliminated by a perifibrotic inflammatory zone. The content and biosynthesis of collagen and noncollagenous proteins were studied in both fibrotic and perifibrotic zones after incubation of the biopsies with (/sup 14/C)proline or (/sup 35/S)methionine for 24 hr. Cells of perifibrotic and fibrotic regions synthesize about 10 times more proteins than those in the nonirradiated muscle. When compared to normal muscle tissue, our results indicate an important increase in collagen content and biosynthesis in fibrotic tissue. The increase in collagen biosynthesis in the irradiated tissue is more pronounced for type III collagen than for type I collagen. Biosynthesis of type III and type I collagens increases 20- and 10-fold, respectively, compared to the normal muscle. Type I to III collagen ratio in irradiated tissue decreases from 2.3 in normal tissue to 1.1 in fibrotic tissue. Histological examination of the biopsies as well as the protein pattern by polyacrylamide gel electrophoresis show striking differences in the perifibrotic and fibrotic areas as compared to the normal muscular tissue with a progressive disappearance of the myotubes replaced by a dense sclerotic tissue. The results indicate that the perifibrotic inflammatory area is engaged in a remodeling process and that the fibrotic tissue remains active in the neosynthesis of the extracellular matrix macromolecules with a high proportion of type III collagen. This high biosynthetic activity of the irradiated tissue may explain the pseudosarcomatous character of the radiation-induced lesions.

• Research Organization: Universite Paris Val de Marne, Creteil (France)
• OSTI ID: 6922606
• Journal Information: Exp. Mol. Pathol.; (United States), Journal Name: Exp. Mol. Pathol.; (United States) Vol. 48:3; ISSN EXMPA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COLLAGEN
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROSIS
GAMMA RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFLAMMATION
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MUSCLES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SCLEROPROTEINS
SKIN
SULFUR 35
SULFUR ISOTOPES
SWINE
SYMPTOMS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES