Heparin fragments modulate the collagen phenotype of fibroblasts from radiation-induced subcutaneous fibrosis

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Experimental and Molecular Pathology; (USA)

DOI:https://doi.org/10.1016/0014-4800(89)90012-9· OSTI ID:5128866

el Nabout, R; Martin, M; Remy, J; Robert, L; Lafuma, C ^[1]

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1. Laboratoire de Biochimie de Tissu Conjonctif, Creteil (France)

Acute local gamma irradiation of porcine skin induces, as in human skin, an extensive and mutilating sclerosis characterized by continuous expansion of the fibrosis invading the adjacent muscle and by accumulation of the macromolecular components of the extracellular matrix. Collagen synthesis, content, and types were studied in the presence of heparin fragments (100 micrograms/10(6) cells) in the culture medium, by measuring the incorporation of the radiolabeled precursor (3H)proline into confluent primary cultures of porcine fibroblasts obtained from normal and irradiated fibrotic dermis. Enhancement in collagen biosynthesis and deposition and preferential increase in collagen type III synthesis were observed in fibrotic fibroblast cultures when compared to those in normal dermis fibroblasts. The total collagen synthesis and the rate of collagen hydroxylation appear unmodified by heparin fragments both in normal and in fibrotic fibroblast cultures. But heparin fragments induce a 10- and 2-fold decrease, respectively, in collagen type III and type V syntheses by fibrosis fibroblasts. As only minor effects upon collagen type III and V are observed in cultures of normal dermis fibroblasts, these results highly suggest that heparin fragments are capable of specifically modulating the collagen phenotype of fibroblasts derived from radiation-induced dermis fibrosis and thus are able to regulate the fibrotic process.

• OSTI ID: 5128866
• Journal Information: Experimental and Molecular Pathology; (USA), Journal Name: Experimental and Molecular Pathology; (USA) Vol. 51:2; ISSN 0014-4800; ISSN EXMPA
• Country of Publication: United States
• Language: English

Related Subjects

550901 -- Pathology-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CULTURES
COLLAGEN
CONNECTIVE TISSUE CELLS
DISEASES
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
FIBROSIS
GAMMA RADIATION
HEMATOLOGIC AGENTS
HEPARIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
MAMMALS
MUCOPOLYSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHENOTYPE
POLYSACCHARIDES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SACCHARIDES
SCLEROPROTEINS
SKIN
SKIN DISEASES
SOMATIC CELLS
SWINE
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES