Inhibition of collagen production in scleroderma fibroblast cultures by a connective tissue glycoprotein extracted from normal dermis
Journal Article
·
· J. Invest. Dermatol.; (United States)
It was shown in a previous paper that a connective tissue glycoprotein (CTGP) extracted from normal rabbit dermis was able to inhibit total protein and collagen syntheses by normal dermis fibroblast cultures. In the present study, the effects of CTGP on scleroderma fibroblasts were investigated. (/sup 14/C)Proline incorporation into total proteins of the supernatant was not significantly different from that found in controls. By contrast, the amount of collagen, expressed as percentage of total secreted protein, was far higher in scleroderma cultures than in normal ones (14.4% +/- 6.0% vs 4.6% +/- 0.9%). Addition of CTGP to the medium induced a concentration-dependent inhibition of (/sup 14/C)proline incorporation into proteins from both control and scleroderma cells. In control cultures, no significant decrease of the percentage of collagen was observed, but over 60 micrograms/ml, both cytotoxic effects and inhibition of protein synthesis occurred. In scleroderma cultures, the inhibition was twice as effective on collagen as on noncollagen protein synthesis. The inhibition of collagen secretion was not related either to changes in collagen hydroxylation or to the intracellular catabolism of newly synthesized procollagen.
- Research Organization:
- Centre Hospitalier-Universitaire, Reims, France
- OSTI ID:
- 5023925
- Journal Information:
- J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 2; ISSN JIDEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATABOLISM
COLLAGEN
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
FIBROBLASTS
GLUCOPROTEINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXYLATION
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RABBITS
SACCHARIDES
SCLEROPROTEINS
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATABOLISM
COLLAGEN
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
FIBROBLASTS
GLUCOPROTEINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXYLATION
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RABBITS
SACCHARIDES
SCLEROPROTEINS
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES