Effects of cis-4-hydroxy-L-proline, an inhibitor of Schwann cell differentiation, on the secretion of collagenous and noncollagenous proteins by Schwann cells

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90318-7· OSTI ID:5322304

Eldridge, C F; Bunge, R P; Bunge, M B ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (USA)

The proline analog cis-4-hydroxy-L-proline (CHP) was previously shown to inhibit both Schwann cell (SC) differentiation and extracellular matrix (ECM) formation in cultures of rat SCs and dorsal root ganglion neurons. The authors confirmed that CHP inhibits basal lamina formation by immunofluorescence with antibodies to laminin, type IV collagen, and heparan sulfate proteoglycan. In order to test the hypothesis that CHP inhibits SC differentiation by specifically inhibiting the secretion of collagen. Cultures grown in the presence or absence of CHP were metabolically labeled with ({sup 3}H)leucine and the media were analyzed for relative amounts of (a) collagenous and noncollagenous proteins by assay with bacterial collagenase and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), or (b) triple-helical collagen by pepsin digestion followed by SDS-PAGE. The results indicate that although CHP inhibited the accumulation of secreted collagen in the culture medium and disrupted collagen triple-helix formation, it also significantly inhibited the accumulation of secreted noncollagenous proteins in the medium. They conclude that CHP does not act as a specific inhibitor of collagen secretion in this system, and thus data from these experiments cannot be used to relate SC collagen production to other aspects of SC differentiation. They discuss the evidence for and against specificity of CHP action in other systems.

• OSTI ID: 5322304
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 174:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CULTURES
CELL DIFFERENTIATION
COLLAGEN
ELECTROPHORESIS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYPROLINE
INHIBITION
LABELLING
LEUCINE
MAMMALS
NERVE CELLS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
PYRROLIDINES
RATS
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES