Long-term overproduction of collagen in radiation-induced fibrosis

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577976· OSTI ID:6143822

Remy, J; Wegrowski, J; Crechet, F; Martin, M; Daburon, F ^[1]

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1. Commissariat a l'Energie Atomique, CEN Saclay, Gif-sur-Yvette (France)

Collagen metabolism was investigated in the fibrotic tissue which developed in pig thigh muscle 6 to 15 months after acute gamma irradiation. During this period, total collagen deposits in the fibrotic tissue increased 10-fold compared to the healthy muscle tissue. These deposits were composed mainly of type I and III collagen, and the type I/type III ratio was lower in the fibrotic than in the muscle tissue. Small pieces of both fibrotic and muscle tissue were incubated with (14C)proline. The (14C)hydroxyproline content of the fibrotic tissue reflected large concomitant increases in the synthesis of total collagen, mainly of types I and III, which rose 14- and 17-fold, respectively. Similarly, the level of type I and type III procollagen mRNAs rose 9- and 5-fold, respectively, in the fibrotic tissue versus the muscle tissue. These results suggest that procollagen gene transcription or RNA maturation in the cell nuclei is activated in the fibrotic tissue. The possibility that such activation is due to the long-term inflammatory state of this tissue is discussed.

• OSTI ID: 6143822
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 125:1; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550901 -- Pathology-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
COLLAGEN
DELAYED RADIATION EFFECTS
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
FIBROSIS
GAMMA RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MESSENGER-RNA
METABOLISM
MUSCLES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RNA
SCLEROPROTEINS
SWINE
TRACER TECHNIQUES
TRANSCRIPTION
VERTEBRATES