Detection of TEM-induced reciprocal translocations in F/sub 1/ sons of CD-1 male mice: comparison of sequential fertility evaluation and cytogenetic analysis
To determine the positive and negative classification error rates associated with the HTA in our laboratory, F/sub 1/ sons of TEM-exposed CD-1 male mice were evaluated by the sequential fertility method with subsequent cytogenetic analysis. Males who sired three litters of size 10 or less when mated to primiparous females from either the B6C3F/sub 1/ or the BCF/sub 1/ strain were classified as partial steriles. When meiotic chromosomes analyses revealed the presence of at least two cells containing multivalent figures, males were classified as translocation heterozygotes. When the fertility evaluation and the cytogenetic analysis were compared, normal fertility was observed on 5 of 83 (6.02%) translocation-bearing F/sub 1/ males mated to B6C3F/sub 1/ tester females and on 3 of 83 (3.61%) F/sub 1/ males mated to BCF/sub 1/ tester females. Thus, the false-negative error rates were 6.02% and 3.61% with these two tester strains. Multivalent figures were not observed in the meiotic chromosomes of 410 F/sub 1/ males. The false-positive error rates with these two tester strains were 2.93% for the B6C3F/sub 1/ strain and 1.71% for the BCF/sub 1/ strain. The results indicate that nonzero error rates, both false-positive and false-negative, are associated with the sequential mating method HTA. In addition, the magnitude of these error rates was influenced not only by the tester female strain but also by the genotype of the F/sub 1/ male.
- Research Organization:
- National Center for Toxicological Research, Jefferson, AR (USA)
- OSTI ID:
- 6922027
- Journal Information:
- Environ. Mol. Mutagen.; (United States), Vol. 11:2
- Country of Publication:
- United States
- Language:
- English
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ALKYLATING AGENTS
GENETIC EFFECTS
CHROMOSOMAL ABERRATIONS
CYTOLOGY
FERTILITY
MALES
MEIOSIS
MICE
MUTATION FREQUENCY
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
CELL DIVISION
MAMMALS
MUTATIONS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology