Translocation X; Y system for detecting meiotic nondisjunction and chromosome breakage in males of Drosophila melanogaster

Journal Article · Wed Aug 01 00:00:00 EDT 1979 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.793153· OSTI ID:6739153

Foureman, P A

A nondisjunction and chromosome breakage screening system involves an X;Y translocation with the short arm of the Y (Ys), marked with the wild type allele of yellow, attached to the distal end of an X carrying the recessive marker y; and the long arm of the Y chromosome (YL), marked with the dominant locus Bar of Stone (BS), attached to the proximal end of the X. A female tester strain carrying normal chromosomes homozygous for the yellow allele is employed in the mating scheme. Following normal disjunction in the male, all zygotes will die as a result of genetic imbalance. Thus all survivors can be classified as exceptions arising from: (1) nondisjunction in the female; (2) gross deletion of the paternal X;Y chromosome; (3) complete loss of the paternal X;Y chromosome; or (4) primary meiotic nondisjunction in the male. Positive results have been obtained with the known mutagens EMS and x-radiation.

• Research Organization: Univ. of Wisconsin, Madison, WI (United States)
• OSTI ID: 6739153
• Journal Information: Environ. Health Perspect.; (United States), Vol. 31
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMOSOME BREAKAGE
RADIOINDUCTION
CHROMOSOME LOSSES
EMS
SIDE EFFECTS
NON-DISJUNCTION
X CHROMOSOME
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
X RADIATION
Y CHROMOSOME
CHROMOSOMAL ABERRATIONS
DROSOPHILA
MALES
MUTAGEN SCREENING
ANIMALS
ARTHROPODS
CHROMOSOMES
ELECTROMAGNETIC RADIATION
ESTERS
FLIES
FRUIT FLIES
HETEROCHROMOSOMES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
LOSSES
MUTAGENS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SCREENING
SULFONIC ACID ESTERS
560153* - Radiation Effects on Animals- Invertebrates- (-1987)