Translocation X; Y system for detecting meiotic nondisjunction and chromosome breakage in males of Drosophila melanogaster

Journal Article · Wed Aug 01 04:00:00 EDT 1979 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.793153· OSTI ID:6739153

Foureman, P A

A nondisjunction and chromosome breakage screening system involves an X;Y translocation with the short arm of the Y (Ys), marked with the wild type allele of yellow, attached to the distal end of an X carrying the recessive marker y; and the long arm of the Y chromosome (YL), marked with the dominant locus Bar of Stone (BS), attached to the proximal end of the X. A female tester strain carrying normal chromosomes homozygous for the yellow allele is employed in the mating scheme. Following normal disjunction in the male, all zygotes will die as a result of genetic imbalance. Thus all survivors can be classified as exceptions arising from: (1) nondisjunction in the female; (2) gross deletion of the paternal X;Y chromosome; (3) complete loss of the paternal X;Y chromosome; or (4) primary meiotic nondisjunction in the male. Positive results have been obtained with the known mutagens EMS and x-radiation.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 6739153
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 31; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560153* -- Radiation Effects on Animals-- Invertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CHROMOSOME LOSSES
CHROMOSOMES
DROSOPHILA
ELECTROMAGNETIC RADIATION
EMS
ESTERS
FLIES
FRUIT FLIES
HETEROCHROMOSOMES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
LOSSES
MALES
MUTAGEN SCREENING
MUTAGENS
MUTATIONS
NON-DISJUNCTION
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SCREENING
SIDE EFFECTS
SULFONIC ACID ESTERS
X CHROMOSOME
X RADIATION
Y CHROMOSOME