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1,25-dihydroxyvitamin D sub 3 up-regulates the 1,25-dihydroxyvitamin D sub 3 receptor in vivo

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America; (USA)
; ; ;  [1]
  1. Univ. of Wisconsin, Madison (USA)
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The level of mRNA encoding the 1,25-dihydroxyvitamin D{sub 3} receptor in the intestine of vitamin D-deficient rats given 1,25-dihydroxyvitamin D{sub 3} was determined by Northern blot analysis using a {sup 32}P-labeled cDNA probe to the 1,25-dihydroxyvitamin D{sub 3} receptor. mRNA levels increased 10-fold above deficiency levels at 6 and 12 hr after an intravenous dose of 1,25-dihydroxyvitamin D{sub 3}, returning to predosing levels at 24 hr. Total receptor protein level determined by an immunoradiometric assay was increased 2-fold at 12 hr. No change in unoccupied receptor levels determined by ligand-binding assay was observed during this period. These results suggest that 1,25-dihydroxyvitamin D{sub 3} increases receptor mRNA and total receptor level to maintain constant levels of unoccupied receptor.

OSTI ID:
6921575
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:24; ISSN 0027-8424; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CHEMISTRY
CHOLECALCIFEROL
DAYS LIVING RADIOISOTOPES
DNA
HYDROGEN COMPOUNDS
ISOTOPES
LIGANDS
LIGHT NUCLEI
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOASSAY
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RNA
TRITIUM COMPOUNDS
VITAMIN D
VITAMINS