Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Loss of Ia-bearing splenic adherent cells after whole body ultraviolet irradiation

Journal Article · · J. Immunol.; (United States)
OSTI ID:6920290
; ; ; ;
Daily uv irradiation of mice results in a marked decrease in the antigen-presenting capability of SAC from these mice after 1 wk of uv exposure. To directly examine this cell population, we developed a technique for purifying SAC that involves passing mouse splenocytes through two cycles of glass adherence with an intervening incubation on rabbit anti-mouse Ig-coated dishes. SAC from externally uv irradiated mice prepared by this method, when pulsed with antigen, activate primed T cells to proliferate much less efficiently than SAC from normal mice. Both the proportion and absolute number of Ia-bearing cells in this purified SAC population from uv irradiated mice are considerably smaller than that seen in similarly prepared populations from normal mice. Previous adjuvant immunization was shown to override functional defects elicited by external uv irradiation. This demonstration of a uv irradiation induced selective loss of Ia bearing splenic adherent cells and the functional consequences of this loss provide further evidence for the importance of Ia-bearing accessory cells in antigen presentation of T dependent antigens, and provides insight into the origin of the immunologic defects induced by whole body uv irradiation.
Research Organization:
Harvard Medical School, Boston, MA
OSTI ID:
6920290
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 125:6; ISSN JOIMA
Country of Publication:
United States
Language:
English

Similar Records

Immunologic effects of whole-body ultraviolet irradiation: selective defect in splenic adherent cell function in vitro
Journal Article · Thu May 01 00:00:00 EDT 1980 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:6688698
Immunologic effects of whole-body ultraviolet (uv) irradiation
Journal Article · Tue Sep 15 00:00:00 EDT 1981 · Cell. Immunol.; (United States) · OSTI ID:5922381
Immunologic effects of whole body ultraviolet (uv) irradiation. II. Defect in splenic adherent cell antigen presentation for stimulation of T cell proliferation. [Mice]
Journal Article · Mon Sep 01 00:00:00 EDT 1980 · J. Immunol.; (United States) · OSTI ID:5072166

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FAR ULTRAVIOLET RADIATION
IMMUNOSUPPRESSION
IRRADIATION
LEUKOCYTES
LOSSES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SPLEEN CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
WHOLE-BODY IRRADIATION