Deletion of stably integrated DNA is suppressed by cadmium and zinc
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Stanford Univ., CA (USA)
The bacterial xanthine-guanine phosphoribosyltransferase (GPT) gene was fused to a metal-responsive promoter and transfected into a murine cell line. Clonal transformants harboring metal-responsive or nonresponsive GPT genes (using a thymidine kinase promoter) were then studied for the loss of transfected gene function either during periods of constitutive expression or during periods of induced activity. Nontoxic levels of cadmium and zinc markedly reduced the frequency of mutagenesis in all transfected lines irrespective of transcriptional status. A survey of 17 GPT{sup {minus}} clones derived from two original transfectants showed partial or complete excisions of the transfected gene in every case. These studies show that quantities of cadmium and zinc that induce metallothioneins also suppress the incidence of deletions in murine cells.
- OSTI ID:
- 6919312
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:23; ISSN PNASA; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CADMIUM COMPOUNDS
COLONY FORMATION
DNA
ENZYME ACTIVITY
ENZYMES
GENE MUTATIONS
GENE RECOMBINATION
GENE REPRESSORS
GENES
GLYCOSYL TRANSFERASES
INHIBITION
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
MICE
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
RODENTS
TRANSFERASES
VERTEBRATES
ZINC COMPOUNDS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CADMIUM COMPOUNDS
COLONY FORMATION
DNA
ENZYME ACTIVITY
ENZYMES
GENE MUTATIONS
GENE RECOMBINATION
GENE REPRESSORS
GENES
GLYCOSYL TRANSFERASES
INHIBITION
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
MICE
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
RODENTS
TRANSFERASES
VERTEBRATES
ZINC COMPOUNDS