Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells
Chinese hamster ovary cells were transfected by human DNA ligated to the bacterial gpt (xanthine-guanine-phosphoribosyltransferase) gene which was used either in its native form or after partial inactivation with methylnitrosourea. The gpt+ transfectants were screened for resistance to high doses of N-methyl-N'-nitro-N-nitrosoguanidine. Using this approach, we showed that Chinese hamster ovary cells can acquire N-methyl-N'-nitro-N-nitrosoguanidine resistance upon transfection with DNA from diploid human fibroblasts, that this resistance is transferable by secondary transfection and is specific for methylating mutagens, and that it is not caused by increased removal of O6-methylguanine, 3-methyladenine, and 7-methylguanine from DNA.
- Research Organization:
- German Cancer Research Center, Heidelberg, Germany, F.R.
- OSTI ID:
- 6271647
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 7:5; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Cells
& Tissue Culture
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTIONS
CHO CELLS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYMES
FIBROBLASTS
GENES
GENETIC EFFECTS
GENETIC ENGINEERING
GUANINE
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MAN
NITROSO COMPOUNDS
NITROSOUREAS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERIZATION
PRIMATES
PURINES
RADIATIONS
RECOVERY
REPAIR
RODENTS
SENSITIVITY
SOMATIC CELLS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES