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Regulation of the capacity for O/sup 6/-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridization

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.2.8.904· OSTI ID:6030584
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Hybrids were made between a ouabain-resistant, thioguanine-resistant human lymphoma line able to remove O/sup 6/-methylguanine from its DNA (Mex/sup +/) and human lymphoblastoid lines deficient in this capability (Mex/sup -/). The formation of hybrids was confirmed by chromosomal analysis. Hybrid cells had an O/sup 6/-methylguanine removal capacity per mole of guanine about one third to one half that of the Mex/sup +/ parents, i.e., about the same per cell. Cell hybrids removed the same amount of the alkylation adduct 3-methyladenine as did their parents per mole of guanine, i.e., about twice as much per cell. Although the cell hybrids had intermediate resistance to the cytotoxic action of N-methyl-N'-nitro-N-nitrosoguanidine used to induce O/sup 6/-methylguanine and 3-methyladenine, there is evidence that the ability to remove O/sup 6/-methylguanine and resistance to the cytotoxic effect of N-methyl-N'-nitro-N-nitrosoguanidine are dissociable characteristics.
Research Organization:
Dept. of Microbiology and Committee on Genetics, The Univ. of Chicago, Chicago, IL 60637
OSTI ID:
6030584
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:8; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ADDUCTS
ADENINES
AMINES
ANIMAL CELLS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBONIC ACID DERIVATIVES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMICAL REACTIONS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DNA ADDUCTS
DNA REPAIR
DNA-ASE
DRUGS
ENDONUCLEASES
ENZYMES
ESTERASES
GENE REGULATION
GENETIC MAPPING
GLYCOSIDES
GUANIDINES
GUANINE
HETEROCYCLIC COMPOUNDS
HUMAN POPULATIONS
HYBRIDIZATION
HYDROLASES
HYDROXY COMPOUNDS
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOMAS
MAPPING
MATERIALS
METHYLATION
NEOPLASMS
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
PHOSPHODIESTERASES
POPULATIONS
PURINES
REACTION KINETICS
RECOMBINANT DNA
RECOVERY
REPAIR
SENSITIVITY
SOMATIC CELLS
STROPHANTHINS