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Depletion of pituitary prolactin by cysteamine is due to loss of immunological activity

Journal Article · · Endocrinology; (United States)
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The mechanism by which cysteamine reduces the prolactin(PRL) content of pituitary cells was studied in primary cultures of estradiol-induced pituitary tumors in Fischer 344 rats. The PRL content of these cells was effectively decreased by cysteamine, with an IC50 of 0.2 mM. Cells previously labeled with (/sup 3/H)leucine were exposed to cysteamine (0.25 mM), and the intracellular content of (/sup 3/H)PRL was measured by immunological or nonimmunological means, that is by immunoprecipitation and electrophoresis or by electrophoresis alone. The intracellular concentration of immunoreactive (/sup 3/H)PRL was reduced by 53% by cysteamine, whereas (/sup 3/H)PRL quantified by electrophoresis alone was not significantly affected. Our data indicate that cysteamine reduces the PRL content of pituitary tumor cells by causing the loss of its immunoreactivity.

Research Organization:
Department of Pharmacology, Yale University School of Medicine, New Haven, CT
OSTI ID:
6917495
Journal Information:
Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 114:3; ISSN ENDOA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CULTURES
DRUGS
ELECTROPHORESIS
ESTRADIOL
ESTRANES
ESTROGENS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
LTH
MAMMALS
MEA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SIDE EFFECTS
STEROID HORMONES
STEROIDS
SYNTHESIS
THIOLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES