Cysteamine depletes prolactin (PRL) but does not alter the structure of PRL-containing granules in the anterior pituitary
Journal Article
·
· Endocrinology; (United States)
Cysteamine causes a profound depletion of PRL in the anterior pituitary and in the systemic circulation, as measured by RIA and bioassay. However, electron microscopic study of PRL-containing cells in rat anterior pituitary does not reveal changes in secretory granule or cytoplasmic structure during the interval of depressed PRL content and of subsequent recovery to normal levels. In contrast to the results obtained by RIA, PRL-like immunoreactivity as detected by immunocyto-chemistry is present and similar to that of control preparations after cysteamine administration. We suggest that cysteamine alters PRL structure in secretory granules, probably by interacting with the disulfide bonds of PRL, thereby altering bioactivity and immunoreactivity. The presence of cysteamine-altered PRL in secretory granules does not seem to trigger degradation of granules by the lysosomal system.
- Research Organization:
- Massachusetts General Hospital, Boston
- OSTI ID:
- 5765468
- Journal Information:
- Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 115:4; ISSN ENDOA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CHEMISTRY
CYTOCHEMISTRY
DRUGS
ELECTRON MICROSCOPY
ENDOCRINE GLANDS
GLANDS
GONADOTROPINS
HORMONES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LTH
MEA
MICROSCOPY
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOPROTECTIVE SUBSTANCES
THIOLS
TRACER TECHNIQUES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CHEMISTRY
CYTOCHEMISTRY
DRUGS
ELECTRON MICROSCOPY
ENDOCRINE GLANDS
GLANDS
GONADOTROPINS
HORMONES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LTH
MEA
MICROSCOPY
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOPROTECTIVE SUBSTANCES
THIOLS
TRACER TECHNIQUES