Depletion of bovine pituitary prolactin by cysteamine involves a thiol:disulfide mechanism
Journal Article
·
· Endocrinology; (United States)
OSTI ID:5777214
Cysteamine (2-aminoethanethiol (CySH)) reduces measurable PRL concentrations in vivo and in vitro. Since secretion is also inhibited, CySH may block conversion from a poorly assayable hormone storage form(s) to readily assayable, releasable PRL. This would represent a previously unrecognized mechanism for secretory regulation. We undertook the present study to identify the sites involved in the loss of measurable PRL (depletion) induced by cysteamine. The disulfide cystamine was ineffective on secretory granules unless combined with reduced glutathione, indicating the generation of the active CySH-thiol form. Pretreatment of granules with thiol-blocking agents resulted in dose-dependent enhancement of CySH inhibition, achieving nearly complete inhibition with 5 mM iodoacetamide. In contrast, pretreatment with reduced glutathione or dithiothreitol, respectively, impaired or abolished the CySH effect. These data suggest that the mechanism by which CySH causes PRL depletion is mediated by granule disulfides and the -SH of CySH. The regulation of thiol:disulfide equilibria appears to be an important determinant of the detectability of PRL storage forms and of their secretion.
- Research Organization:
- Univ. of Rochester School of Medicine and Dentistry, NY
- OSTI ID:
- 5777214
- Journal Information:
- Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 115:4; ISSN ENDOA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cysteamine inhibition of prolactin immunoassayability and secretion: studies with aminothiophenols and other analogs
Cysteamine, zinc, and thiols modify detectability of rat pituitary prolactin: a comparison with effects on bovine prolactin suggests differences in hormone storage
Cysteamine depletes prolactin (PRL) but does not alter the structure of PRL-containing granules in the anterior pituitary
Journal Article
·
Sat Sep 01 00:00:00 EDT 1984
· Endocrinology; (United States)
·
OSTI ID:6350324
Cysteamine, zinc, and thiols modify detectability of rat pituitary prolactin: a comparison with effects on bovine prolactin suggests differences in hormone storage
Journal Article
·
Fri Feb 28 23:00:00 EST 1986
· Metab., Clin. Exp.; (United States)
·
OSTI ID:5527211
Cysteamine depletes prolactin (PRL) but does not alter the structure of PRL-containing granules in the anterior pituitary
Journal Article
·
Mon Oct 01 00:00:00 EDT 1984
· Endocrinology; (United States)
·
OSTI ID:5765468
Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CATTLE
CHEMISTRY
DISULFIDES
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
GLUTATHIONE
GONADOTROPINS
HORMONES
LTH
MAMMALS
MEA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PITUITARY GLAND
PITUITARY HORMONES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RUMINANTS
THIOLS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CATTLE
CHEMISTRY
DISULFIDES
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
GLUTATHIONE
GONADOTROPINS
HORMONES
LTH
MAMMALS
MEA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PITUITARY GLAND
PITUITARY HORMONES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RUMINANTS
THIOLS
VERTEBRATES