A muscle-specific intron enhancer required for rescue of indirect flight muscle and jump muscle function regulates Drosophila tropomyosin I gene expression

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.4.1901· OSTI ID:6913901

Schultz, J A; Gremke, L; Storti, R V ^[1]; Tansey, T ^[2]

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1. University of Illinois of Medicine, Chicago (United States)
2. Georgetown University, Washington, D.C., VA (United States)

The control of expression of the Drosophila melanogaster tropomyosin I (TmI) gene has been investigated by P-element transformation and rescue of the flightless TmI mutant strain, Ifm(3)3. To localize cis-acting DNA sequences that control TmI gene expression, Ifm(3)3 flies were transformed with P-element plasmids containing various deletions and rearrangements of the TmI gene. The effects of these mutations on TmI gene expression were studied by analyzing both the extent of rescue of the Ifm(3)3 mutant phenotypes and determining TmI RNA levels in the transformed flies by primer extension analysis. The results of this analysis indicate that a region located within intron 1 of the gene is necessary and sufficient for directing muscle-specific TmI expression in the adult fly. This intron region has characteristics of a muscle regulatory enhancer element that can function in conjunction with the heterologous nonmuscle hsp70 promoter to promote rescue of the mutant phenotypes and to direct expression of an hsp70-Escherichia coli lacZ reporter gene in adult muscle. The enhancer can be subdivided further into two domains of activity based on primer extension analysis of TmI mRNA levels and on the rescue of mutant phenotypes.

• OSTI ID: 6913901
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:4; ISSN MCEBD4; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTHROPODS
BIOLOGICAL FUNCTIONS
DIPTERA
DNA SEQUENCING
DROSOPHILA
FLIES
FRUIT FLIES
GENE MUTATIONS
GENE REGULATION
INSECTS
INVERTEBRATES
MUSCLES
MUTATIONS
PHENOTYPE
STRUCTURAL CHEMICAL ANALYSIS
TROPOMYOSIN